Determination of Amoxycillin capsules (500mg) by UV Spectrophometric method

1.0 OBJECTIVE                    

To provide the procedure for the determination of Amoxycillin capsules (500mg) by UV Spectrophometric method

2.0 SCOPE

This procedure is Applicable to the determination of Amoxycillin capsules (500mg) by UV Spectrophometric method

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE          

5.1 Label Claim: 

Each Hard gelatin capsule Contains:

Amoxycillin  Trihydrate  IP

Equivalent to Amoxycillin ……………….500mg

5.2 Assay Method: 

5.2.1 Preparation of standard solution:

Dissolve 100mg of amoxycillin trihydrate deionized water, with using ultra sonic devise for dissolving the compound and diluting to 100mL in a volumetric flask. Futher dilute 1 ml of the above to 100 ml with deionized water.

5.2.2 Preparation of Sample solution:

Weight powder twenty capsules and take the powder equivalent to 100mg  was accurately weighed, dissolved in 25ml of deionized water, filtered through Whatmann filter paper No: 41 further diluted to 100ml with deionized water,.

5.2.3 Preparation of Final Standard Solution

In a 25 mL volumetric flask, 1ml of standard solution, add 0.5 mL HCl (0.01 N), 1.0ml of Sodium Nitrate(0.2%), and 1.0 mL of sulphanilic acid (0.5%), 1.0 mL of sodium carbonate solution(0.5 N), then make up the volume with deionized water.

5.2.4 Prepare the Sample Solution as per 5.2.3

5.2.5 Measure the absorbance at about 455nm

5.2.6 Calculate the content by comparison.

Sample absorbance    x  Standard weight  x Assay x 1 x Average weight of the capsule = 

Standard absorbance  x  Sample Weight   x   100   x1.148  

= ____________x100

         LC 

LC: Label Claim

6.0 ANNEXURES

Nil