Settle Plate method for Environmental Monitoring in Microbiology Lab

1.0 OBJECTIVE

To describe a Procedure for Environmental Monitoring by Settle Plate method at Microbiology Lab.

2.0 SCOPE

This procedure is applicable the Procedure of Environmental Monitoring by Settle Plate Method at pharma area and Microbiology area.

3.0 RESPONSIBILITY

3.1 Microbiologist /Officer is responsible for the execution. 

3.2 HOD or designee is responsible for review, effective implementation and compliance to the Environmental Monitoring by Settle Plate for execution.             

4.0 PROCEDURE 

4.1 Prepare Soyabean Casein Digest agar (SCDA) and Sabouraud Dextrose agar (SDA) as per current version of SOP No.:QCP/019 and add 10ml glycerol per 1000ml of media.

4.2 Aseptically dispense the media in Petri dishes.

4.3 After solidification invert and incubate the plates for 24-48 hours at 30-35°C.

4.4 Carry the plates in the Petri cans for plate exposing to the respective area.

4.5 Follow the entrance procedure available at that place and enter into the respective area accordingly. 

4.6 Mark the Plates as per Point Name and according to respective area 

4.7 Wear the hand gloves and clean the surface where plate has to be exposed by 70% isopropyl alcohol.

4.8 Expose the plates at the locations mentioned as per the Appendix. 

4.9 Open the plate carefully and keep the lid beside and expose the plates.

4.10 After completion close the plate by lid and carry the plates in Petri cans to the microbiology lab. 

4.11 Incubate both the Soyabean Casein Digest Agar plates at 30-35°C for 3 days and Sabouraud Dextrose Agar plates at 22-25°C for 5 days.

4.12 Enter the details in miscellaneous register and assign Inspection lot number.

4.13 After incubation observe the plates for colonies and record the details as per the Attachment#1(Environmental monitoring by plate exposing.)

4.14 Frequency: Every week in microbiology controlled area and every month in Production area.