1.0 OBJECTIVE
To provide the procedure for calculating the Resolution Calculation as per EP
2.0 SCOPE
This procedure is
3.0 RESPONSIBILITY
3.1 QC-Chemist
4.0 ACCOUNTABILITY
4.1 Executive – Quality control
4.2 Head – Quality control
5.0 PROCEDURE
5.1 In liquid chromatography, the degree of resolution (Rs) is the most important part of chromatographic separation. Regardless of whether the ultimate purpose of chromatography is quantitative and qualitative analysis or sample preparation, it is achieved on the basis of a certain degree of resolution. The formula for calculating the degree of separation is as follows:
5.2 The resolution (Rs) between peaks of 2 components may be calculated from the expression :
5.3 tR1and tR2 = retention times or distances along the baseline from the point of injection to the perpendiculars dropped from the maxima of 2 adjacent peaks,
w1 and w2 = peak widths at half-height.
A resolution of greater than 1.5 corresponds to baseline separation.
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