Estimation of Folic acid tablets (5 mg)

1.0 OBJECTIVE                    

To provide the procedure for the determination of Folic acid tablets (5 mg)

2.0 SCOPE

This procedure is Applicable to the determination of Folic acid tablets (5 mg)

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE          

 5.1 Label Claim: 

Each uncoated tablet Contains:

Folic acid   IP ……………….5mg

5.2 Assay Method: 

5.2.1 Preparation of standard solution:

Dissolve 1.6gm of the Folic acid   in 50ml of double distilled water and further diluted to 100ml with double distilled water. Further dilute 1ml of the above solution into 10ml with double distilled water.

5.2.2 Preparation of Sample solution:

Weight powder twenty tablets and take the powder equivalent to 1.6gm in double distilled water was accurately weighed, dissolved in 50 ml of double distilled water, filtered through Whatmann filter paper No: 41 further diluted to 100ml with double distilled water.

Further dilute 1ml of the above solution into 10ml with double distilled water.

5.2.3 Measure the absorbance at about 250nm

5.2.4 Calculate the content by comparison.

Sample absorbance    x  Standard weight  x Assay x Average weight of the tablet = 

Standard absorbance  x  Sample Weight   x   100     

= ____________x100

         LC 

LC: Label Claim

6.0 ANNEXURES

Nil