Estimation of acetazolamide tablets(250mg) formulation

1.0 OBJECTIVE                    

To provide the procedure for Estimation of acetazolamide tablets formulation

2.0 SCOPE

This procedure is Applicable to the Estimation of acetazolamide tablets formulation

3.0 RESPONSIBILITY

3.1 QC-Chemist 

4.0 ACCOUNTABILITY

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE  

 5.1Label Claim: 

Acetazolamide  IP  250mg

5.2 Assay Method: 

5.2.1 Standard solution:  

Accurately weigh about 100mg of Acetazolamide working standard and transfer it into 100ml of volumetric flask, add 50 ml of methanol, shake well until the material gets dissolved and make up to 100ml with above solution.  

Transfer 1ml of the above solution into 100 ml with methanol.  

5.2.2 Sample solution:

Weigh and powder 20 tablets. Weigh accurately the tablets powder  equivalent to about 100mg of Acetazolamide into 100ml of volumetric flask, add 50ml of methanol, shake well until dissolve the substance or Sonicate for 3 minutes, make up to volume with methanol.

Filter the sample Solution with whatman filter paper and  reject first few ml of the filtrate. 

Transfer 1ml of the above solution into 100 ml with methanol. 

Measure the absorbance at about 263nm using methanol as blank

5.2.3 Calculate the content by comparison.

Sample absorbance    x  Standard weight  x Assay x Average weight of the tablet = 

Standard absorbance  x  Sample Weight   x   100     

= ____________x100

         LC 

LC: Label Claim

6.0 ANNEXURES

Nil