Determination of Ondansetron hydrochloride tablets(4mg) by HPLC method

1.0 OBJECTIVE                    

To provide the procedure for the determination of Ondansetron hydrochloride tablets(4mg) by HPLC method

2.0 SCOPE

This procedure is Applicable to the determination of Ondansetron hydrochloride tablets(4mg)

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE        

  

5.1 Mobile Phase:

50 Volumes of Acetonitrile and 50 Volumes of methanol.

5.2 Standard and sample Preparation:

Weigh accurately about 100mg of Ondansetron hydrochloride working standards in a 100ml volumetric flask and dissolve  and dilute to 100 ml with mobile Phase.

Dilute 1ml of the above solution in to 10 ml with dilute with mobile phase upto the mark.

5.3 Sample Preparation:

Weight powder twenty tablets and take the powder equivalent to 100mg Ondansetron hydrochloride was accurately weighed, dissolve dilute to 100ml with mobile Phase, filtered through Whatmann filter paper No: 41.

Dilute 1ml of the above solution in to 10 ml with dilute with mobile phase upto the mark.

5.4 Chromatographic conditions:

Mode	Isocratic
Flow rate	1.2ml / minute
Wavelength	216nm
Injection volume	20µL
Column	C18, 250*4.6*5µm
Run Time	10 minutes

 5.5 Retention Time:

Ondansetron hydrochloride: 2.64 min

6.0 ANNEXURES

Nil