Assay of Ciprofloxacin IP- 500mg and 250- By UV Spectrophotometric method

1.0 OBJECTIVE                    

To provide the procedure for Assay of Ciprofloxacin  IP- 500mg and 250- By UV Spectrophotometric method

2.0 SCOPE

This procedure is Assay of Ciprofloxacin  IP- 500mg and 250- By UV Spectrophotometric method

3.0 RESPONSIBILITY

3.1 QC-Chemist 

4.0 ACCOUNTABILITY

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE  

5.1 Each Film coated tablet contains:

Ciprofloxacin Hydrochloride IP eq to Ciprofloxacin  500mg / 250 mg

5.2 Assay  

5.2.1 Standard Solution: 

Weigh accurately about 100 mg of ciprofloxacin hydrochloride working standard and transfer it into 100 ml volumetric flask add 60ml of 0.1N Hydrochloric acid and shake well until dissolve then make up to 100 ml with the same solvent. 

5.2.2 Sample Solution:

Weigh accurately  of the powder equivalent to 100 mg of Ciprofloxacin Hydrochloride, transfer to a 100 ml volumetric flask add 60 ml of 0.1N Hydrochloric acid and shake for 15 minutes to dissolve  then make up to 100ml with the same solvent. Filter the solution, reject first few ml of the filtrate.

5.2.3 Procedure:

To 5.0 ml each of sample and standard solutions, add 2.0ml of 2%w/v ferric chloride solution and shake well and allow standing for 2 minutes, dilute to 50ml with 0.1M hydrochloric acid.

5.2.4 Blank: 

5ml of water, 2ml of 2%w/v ferric chloride solution, and make up to 100ml with 0.1M hydrochloric acid.

5.2.5 Measure the absorbance of the solution at about 438nm. Calculate the result by comparison. 

Sample absorbance    x  Standard weight  x Assay x  1  x Average weight of the tablet = 
Standard absorbance  x  Sample Weight   x   100   x    1.16        

= ____________x100

          500

500= Label claim

6.0 ANNEXURES

Nil