Water bath Operation and calibration

 1.0 OBJECTIVE

To lay down the procedure for Operation and calibration of water bath in Microbiology Laboratory.

2.0 SCOPE

This SOP is applicable for Operation and calibration of water bath in Microbiology laboratory.

3.0 BACKGROUND

NIL

4.0 RESPONSIBILITY 

4.1 Microbiology personnel are responsible to follow this SOP.

4.2 Head of the department - QC: To review and ensure compliance of SOP.

4.3 Head of the department - QA: To approve, implement and ensure compliance of SOP.

5.0 PROCEDURE

5.1 Operating procedure

5.1.1    Ensure that the instrument is clean and calibration of temperature indicator is with in  due date of calibration.

5.1.2 Add sufficient quantity of purified water in equipment so as to dip the heating coil completely under water.

5.1.3 Connect the power cord of equipment to electric supply and put on the main  Switch.

5.1.4 The digital temperature controller cum indicator will indicate the actual temperature of water.

5.1.5 Set the desired temperature of the water bath by pressing "SEL" button and adjust the "UP/Down". 

5.1.6 Approximately 1ºC before the set temperature, the heater will start going on and off Heater action is indicated by the LED on the DTC. Allow a few minute for the temperature to stabilize.

5.1.7 If the indicated temperature is less than desired, turn the fine tuning pot on the DTC Clockwise or vice versa.

5.2 Precautions                  

5.2.1 Do not operate without water.

5.2.2 Switch OFF when instrument is not in use.

5.3  Calibration

5.3.1 Calibration after three month.

5.3.2 Set the desired temperature against standard thermometer at 37 ºC, 50 ºC and 90 ºC.

5.3.3 After completing the calibration make entry in the calibration record of water bath as per Annexure– 

5.4  MAINTENANCE

5.4.1 When equipment is under breakdown put the tag stating "UNDER MAINTENANCE" with date and sign of the analyst inform to Head QC.

5.4.2 Then inform to engineering person.

5.4.3 After completion of major repairing / servicing job, calibrate the equipment to ensure the proper working. 

6.0 REFERENCES : nil

7.0 ABBREVIATIONS 

7.1 SOP: Standard operating procedure.

7.2 DTC:  Digital temperature controller.