Calibration of Fourier Transform Infra-Red (FTIR) Spectrophotometer.

1.0 OBJECTIVE :

To provide a procedure for Calibration of Fourier Transform Infra-Red (FTIR) Spectrophotometer.

2.0 SCOPE :

This procedure is applicable to FTIR Spectrophotometer

3.0 RESPONSIBILITY :

Quality Control Chemist and above

4.0 ACCOUNTABILITY:

Quality Control Head

5.0 PROCEDURE :

5.1 PRECAUTIONS

Place the instrument as far as possible away from any strong magnetic or electric field or any electrical appliances generating a high frequency.

Avoid placing it, where severe vibrations exist.

Place the instrument free from dust and corrosive gases.

5.2 CALIBRATION

Change the assembly for calibration instead of assembly for analysis for the calibration.

Handle the calibration assembly very carefully.

Handle the polystyrene film very gently and avoid direct contact to the film.

Verification of Wave Number Scale

Measure the spectrum of polystyrene film 4000 cm-1 to 650 cm-1 after the routine Background correction.

Following bands should appear at the frequencies indicated below and should not deviate from the designated frequency by more than the tolerance indicated.  

Sr. No. Wave-number (cm-1) Acceptable Tolerance (cm-1)

01. 3060.0    ± 1.0 3059.0 –3061.0

02. 2849.5    ± 1.0 2848.5 – 2850.5

03. 1942.9    ± 1.0 1941.9 – 1943.9

04. 1601.2    ± 1.0 1600.2 – 1602.2

05. 1583.0    ±  1.0 1582.0 –1584.0

06. 1154.5     ± 1.0 1153.5 – 1155.5

07. 1028.3    ±  1.0 1027.3 – 1029.3

Control of Resolution Performance

Expand the recorded polystyrene spectrum in the region 3200 cm-1 to   2600 cm-1.

Note down the absorption at wave numbers 2870 cm-1 (tolerance ± 1 cm-1) and 2849.5 cm-1  (tolerance ± 1 cm-1).  

The difference between the absorbances at the absorption minimum at 2870 cm-1 and the absorption maximum at 2849.5 cm-1 is greater than 0.33. 

Expand the polystyrene spectrum in the wave number region 1800 cm-1 to 1400 cm-1.

Note down the absorption at wave numbers the difference the absorption  at 1583 cm-1 (tolerance ± 1 cm-1) and 1589 cm-1 (tolerance ± 1 cm-1).

The difference between the absorbances at the absorption minimum at 1589 cm-1 and the absorption maximum at 1583 cm-1 is greater than 0.08.

Control of Resolution Performance Tolerance Limit

6.0 ABBREVIATIONS:

Abbreviation Expanded form

FTIR Fourier Transform Infra-Red

LED         Light Emitting Diode

PC         Personal Computer

SOP Standard Operating Procedure

cm         Centimeter

7.0 ANNEXURES:

Annex. No.                       Title

01 Calibration of Fourier Transform Infra-Red Spectrophotometer