Micropipette operation and calibration procedure

1.0 OBJECTIVETo describe a procedure for Calibration of Micropipette by using water.

2.0 SCOPE

This procedure is applicable to Calibration of Micropipette and is applicable in Microbiology

3.0 RESPONSIBILITY

3.1 Microbiologist /Officer is responsible for the execution.

3.2 HOD or designee is responsible for review, effective implementation and compliance to the calibration of micropipette for execution.

4.0 PROCEDURE

4.1 Operation

4.1.1 Set the required volume by rotating color knob clockwise and anti clockwise.

4.1.2 Fix the micro tip by applying light pressure.

4.1.3 Press the Plunger and insert the micro tip in the solution to be taken.

4.1.4 Release the plunger to get the required solution.

4.1.5 Transfer the solution by pressing the plunger little more than the normal. Press the dispenser knob to remove the used micro tip.

Note: Use this pipette (100 -1000µl) for volume not less than 100 µl and not more than 1000 µl Use this pipette (1 -10ml) for volume not less than 1ml and not more than 10ml.

4.2 Calibration

4.2.1 Set the volume at 200 µl, 500 µl and 1000µl for micropipette (T -1000µl).

4.2.2 Follow the operation procedure from point no 6.1.2 to 6.1.5.

4.2.3 Take the water at room temperature as per set volume and weigh it in a weighing bottle, which is previously dried and weighed.

4.2.4 Record the reading.

4.2.5 Repeat the analysis for 5 times and take the average.

4.2.6Calculate the volume by dividing the average weight of water with the actual volume from the table given as per the test temperature and record the values in Attachment #1

Temperature	Volume of 1 gm of water	Temperature	Volume of 1 gm of water
15 ⁰ c	1.0020 ml	24 ⁰ c	1.0037 ml
16 ⁰ c	1.0021 ml	25 ⁰ c	1.0039 ml
17 ⁰ c	1.0023 ml	26 ⁰ c	1.0042 ml
18 ⁰ c	1.0025 ml	27 ⁰ c	1.0045 ml
19 ⁰ c	1.0026 ml	28 ⁰ c	1.0047 ml
20 ⁰ c	1.0028 ml	29 ⁰ c	1.0050 ml
21 ⁰ c	1.0030 ml	30 ⁰ c	1.0053 ml
22 ⁰ c	1.0032 ml	31 ⁰ c	1.0056 ml
23 ⁰ c	1.00345 ml	32 ⁰ c	1.0059 ml

4.2.7 Acceptance criteria:

For micropipette 100µl –1000µl

Set volume in Permitted Volume

200-µl 196-204 µl

500-µl 495-505 µl

1000-µl 990-1010µl

Frequency – Quarterly

4.2.8 Precautions :

4.2.8.1 After fixing volume of micropipette don’t rotate the push button.

4.2.8.2 No foreign particle should be present in the tip as this can block the delivery of the liquid and this can result in incorrect volume dispenses.