Growth promotion test for microbial media

1.0 OBJECTIVE

To describe a for growth promotion test of received microbiological media using Standard viable cultures of known organisms obtained from NCIM, IMTECH 

2.0 SCOPE

This procedure is applicable to the procedure for disinfectant efficacy test in company Name.

3.0 RESPONSIBILITY

3.1 Microbiologist /Officer is responsible for the execution. 

3.2 HOD or designee is responsible for review, effective implementation and compliance to the Growth promotion test for execution.                           

4.0 PROCEDURE 

4.1. Each lot of dehydrated medium bearing manufacturers identifying number or each lot of medium prepared from basic ingredients are tested for its growth promoting qualities.

4.2. A minimum amount of 10-100 cells viable suspension is prepared as per current version of SOP and inoculated in the prepared media.

4.3. Different types of media are checked for its growth promotion quality by using relevant viable cultures as per the Attachment # 2 

4.4. Incubate the media in incubator at the temperature specified in Attachment #2 for respective organism growth.

4.5. After the completion of incubation period observe the media for growth of organism inoculated and if growth is confirmed then that media is suitable for further use.

4.6. If the growth is not seen, discard the media container.

4.7. The recovery should not be less than 50%.

4.8. The visual growth obtained is further confirmed by its morphological characteristics.

4.9. Record the growth promotion test details in the Growth promotion test record as per Attachment #1.