Determination of Carisoprodol tablets (350/500mg) by HPLC method.

1.0 OBJECTIVE                    

To provide the procedure for the determination of Carisoprodol tablets (350/500mg) by HPLC method.

2.0 SCOPE

This procedure is Applicable to the determination of Carisoprodol tablets (350/500mg) by HPLC method.

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE          

5.1 Mobile Phase:

Water: acetonitrile (60:40)

5.2 Standard and sample Preparation:

Weigh accurately about 100mg of Carisoprodol working standard in a 100ml volumetric flask and dissolve and dilute to 100 ml with mobile Phase.

Dilute 10ml of the above solution in to 100 ml with dilute with mobile phase upto the mark.

5.3 Sample Preparation:

Weight powder twenty tablets and take the powder equivalent to 100mg Carisoprodol was accurately weighed, dissolve dilute to 100ml with mobile Phase, filtered through Whatmann filter paper No: 41.

Dilute 10ml of the above solution in to 100 ml with dilute with mobile phase upto the mark. 

5.4 Chromatographic conditions:

Mode	Isocratic
Flow rate	1.0ml / minute
Injection volume	20µL
Column	C18, 250*4.6*5µm
Run Time	8 minutes
Temperature	300C
Detector	Refractive index

 5.5 Retention Time:

Carisoprodol: 5.3 min

6.0 ANNEXURES

Nil