Determination of Captopril (25/50mg) tablets by HPLC method

 1.0 OBJECTIVE                    

To provide the procedure for the determination of Captopril (25/50mg) tablets by HPLC method

2.0 SCOPE

This procedure is Applicable to the determination of Captopril (25/50mg) tablets by HPLC method

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE          

5.1 Mobile Phase:

80 Volumes of water, 10 volumes of Acetonitrile, 10 Volumes of Tetrahydrofuran and 0.1 Volumes of methane Sulphonic acid. Adjust the pH of the mobile Phase to 2.5.

5.2 Standard and sample Preparation:

Weigh accurately about 100mg of Captopril working standards in a 100ml volumetric flask and dissolve with 60ml of methanol and dilute to 100 ml with mobile Phase.

Dilute 5ml of the above solution in to 50 ml with dilute with mobile phase upto the mark.

5.3 Sample Preparation:

Weight powder twenty tablets and take the powder equivalent to 100mg Captopril was accurately weighed, dissolved in 60ml of mobile Phase and dilute to 100ml with mobile Phase, filtered through Whatmann filter paper No: 41.

Dilute 5ml of the above solution in to 50 ml with dilute with mobile phase upto the mark.

5.4 Chromatographic conditions:

Mode	Isocratic
Flow rate	1.0ml / minute
Wavelength	220nm
Injection volume	20µL
Column	C8, 250*4.6*5µm
Run Time	10 minutes

 5.5 Retention Time:

Captopril: 3.0 min

6.0 ANNEXURES

Nil