Determination of Atenolol (12.5/25/50/100mg) in tablet dosage form

1.0 OBJECTIVE                    

To provide the procedure for the determination of Atenolol in tablet dosage form

2.0 SCOPE

This procedure is Applicable to the determination of Atenolol in tablet dosage form

3.0 RESPONSIBILITY 

3.1 QC-Chemist 

4.0 ACCOUNTABILITY         

4.1 Executive – Quality control

4.2 Head – Quality control

5.0 PROCEDURE     

5.1 Label Claim: 

Each Uncoated tablet contains:

Atenolol IP 12.5/25/50/100mg

5.2 Assay Method: 

5.21 Preparation of standard solution:

Dissolve 100mg of the Atenolol in 50ml of 0.1N hydrochloric acid  and further diluted to 100ml with  0.1N hydrochloric acid.

Further dilute 1ml of the above solution into 10ml with 0.1N hydrochloric acid.

5.2.2 Preparation of Sample solution:

Weight powder twenty tablets and take the powder equivalent to100mg Atenolol was accurately weighed, dissolved in 100 ml of 0.1N hydrochloric acid, filtered through Whatmann filter paper No: 41. .

Further dilute 1ml of the above solution into 10ml with 0.1N hydrochloric acid.

5.2.3 Measure the absorbance at about 225nm

5.2.4 Calculate the content by comparison.

Sample absorbance    x  Standard weight  x Assay x Average weight of the tablet = 

Standard absorbance  x  Sample Weight   x   100 

= ____________x100

         LC 

LC: Label Claim

6.0 ANNEXURES

Nil